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  • Rouleaux interference - BloodBankTalk - PathLabTalk
    Is it common to have rouleaux interference in gel? Also, there was some disagreement among techs on whether we should do an immediate spin crossmatch only or an AHG crossmatch in these situations
  • Rouleaux or cold auto - BloodBankTalk - PathLabTalk
    We perform our antibody screens at 37C and IAT only (since AABB STD's says methods that detect SIGNIFICANT antibodies) The problem is, that crossmatches are performed at Immediate Spin Cold autos are missed at antibody screening, and the screen is reported as negative When techs crossmatch units,
  • Do you wash your screening cells? For rouleaux? Why?
    I had this patient with multiple myeloma Her antibody screen on gel card had rouleaux reactions for all 3 screening cells I then washed the screening cells six times with warm saline The screen was then negative Forgive me if this is dumb, but why wash the screen cells? Obviously it worked, b
  • can you tell if Rouleaux is present just from looking at the gel card . . .
    I apologize, this may be a stupid question For example, if you are trying to do a gel cross match and the donor cells have Rouleaux, can you tell that from looking at the gel card? If so, can you tell me where I can find some pictures of this reaction Thank you for your time and help in advance
  • anti-A1 or rouleaux? - BloodBankTalk - PathLabTalk
    The forward type is an AB patient, your reverse type looks like you have multiple things going on Because of the negative xm with group O donor red cells and the 1+ reactions with your group A donor red cells, I suspect an anti-A1 However, that 4+ reaction on the B cells cannot be due to the anti-A1 and I highly doubt that it is due to rouleaux; At least from my experience I don't typically
  • saline replacement in serum discrepancy - PathLabTalk
    How do you perform saline replacement when there is serum discrepancy due to rouleaux formantion?From the last step,2 drops of saline is added and resuspend it with red cells does it need to read the agglutination macroscopically?(because iso-antibodies are IgM antibodies) AABB Technical manual d
  • Rouleaux in Gel - BloodBankTalk - PathLabTalk
    Have any other primarily Gel users noticed an increase in Rouleaux in the last 6 months? We have the Provue and do gel manually but have had an increasing need for tube screens because of the pesky rouleaux Rouleaux used to be a rare incovenience but now we are almost seeing one a day Any info
  • Letting tube sit to catch rouleaux or cold? - pathlabtalk. com
    I would consider it to be looking for Zebras, as Malcom said, to want to see rouleaux or a cold-reactive antibody by letting tubes " sit before looking under the microscope" (slightly adulterated version, my apologies) I didn't see any mention of "only for ABO discrepancies" or even "reverse type"
  • Rouleaux - BloodBankTalk - PathLabTalk
    Hello, I currently use Gel as my primary method A tech on the weekend got the following: 3 cell screen: 2+ for allGel panel A (11 cells) 1+ and 2+ reactions with the exception of 2 cells and the Auto control that were neg Gel panel B (11 cells) 1+ and 2+ reactions with the exception of 3 cells t
  • Rouleaux or cold auto - Page 2 - BloodBankTalk - PathLabTalk
    My routine for cold rouleaux encounters is to add two drops of saline and re-spin With weaker rouleaux reactions, the clumping is ususally dispersed If not, I incubate the tubes in a BB refrigerator (top shelf) 2 minutes and re-spin Increased reaction signifies a cold; I re-set up the reaction using plasma prewarmed in the reaction tube If cold incubation reaction did not increase I





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