Highly contiguous assemblies of 101 drosophilid genomes Over 100 years of studies in Drosophila melanogaster and related species in the genus Drosophila have facilitated key discoveries in genetics, genomics, and evolution While high-quality genome assemblies exist for several species in this group,
A field guide to whole‐genome sequencing, assembly and . . . For sequencing, a full genome using a set of different libraries requires ~1 mg of DNA as starting material (~6 μg for short-insert libraries, ~40 μg for 2–10 kb libraries, ~60 μg for >20 kb libraries) Before engaging in genome sequencing, it is thus essential to obtain a large amount of high-quality DNA of the target species
Pangenome graph construction from genome alignments with . . . A pangenome can be represented as a set of variants against a single reference 3, but technological advances in long-read sequencing are now making it possible to produce high-quality de novo
Identification of constrained sequence elements across 239 . . . We combined the resulting contigs together with 52 previously published high-quality primate reference assemblies to create a reference-free whole-genome MSA of 239 primate species with Cactus 21