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  • ZFIN Transgenic Construct: Tg(myl7:H2B-GFP) - Zebrafish Information Network
    Tg(myl7:H2B-GFP) Summary; Plasmid Map; Genomic Features; Transgenics; Sequence Information; Other Construct Pages; Citations
  • The zebrafish issue: 25 years on - The Company of Biologists
    Heart of a 3 days post fertilization zebrafish embryo This myl7:MYL9-mScarlet; myl7:H2B-GFP transgenic zebrafish line labels cardiomyocyte cytoskeleton (myl7:MYL9-mScarlet, depth color-coded) and cardiomyocyte nuclei (myl7:H2B-GFP, gray)
  • Transgenic fluorescent zebrafish lines that have revolutionized . . .
    To visualize overall zebrafish embryonic development, the Tg(actb2:H2B-GFP) line was generated (Table 7) Under the regulation of β-actin promoter, this line expresses fusion proteins of human histone-2 and GFP that localize to chromatin, and has been used to analyze cell cycle length, nuclear architecture and the temporal dynamics of the
  • Defining mononuclear phagocyte distribution and behaviour in the . . .
    Here, we use a combination of transgenic lines to identify distinct MNPs in the zebrafish heart We show that larval macrophage populations have different origins and a sub-population of csf1ra expressing cells are maintained on the surface of the adult heart
  • Live Imaging of Heart Injury in Larval Zebrafish Reveals a . . . - Frontiers
    TUNEL stained injured Tg(myl7:h2b-GFP) heart at 2 hpi Injury site is marked by a loss of nuclear myocardial GFP (white arrowhead) bordered by TUNEL positive cells (magenta)
  • H2B-GFP Sequences (3) - Addgene
    Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and or Sanger results (Addgene Assembled Sequence) Addgene has sequenced portions of this plasmid for verification The results are shown below Leading primers are indicated on the first line of each sequence
  • In vivo analysis of cardiomyocyte proliferation during trabeculation
    We decided to analyze the relative distribution of zebrafish myl7: mVenus-Gmnn + cardiomyocytes at different developmental stages in more detail We crossed the Tg (myl7:mVenus-gmnn) line, which enables one to visualize cycling cardiomyocytes, to the Tg (myl7:MKATE-CAAX) line, which labels cardiomyocyte membranes
  • Alpha-1 adrenergic signaling drives cardiac regeneration via . . .
    7-days-post-fertilization (dpf) myl7:H2B-GFP zebrafish larvae in which cardiomyocyte nuclei were fluorescently labeled with GFP This approach permits the precisely localized and highly reproducible cardiac injury most suitable for pharmacological screening Following injury,wetreated larvaewith differentsym-


















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