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  • Copper-inducible expression system for metabolic engineering . . .
    Subsequently, we demonstrated that the copper-inducible expression system was suitable for the metabolic engineering of E coli toward protocatechuic acid overproduction, and the resulting strain with combined manipulation of the central metabolism via CRISPRi produced 4 12 g L-1 PCA under the optimal copper concentration and induction time
  • Relieving metabolic burden to improve robustness and . . .
    Metabolic burden engineering aims to reduce the impact on genetically modified microorganisms and, consequently, improve both cell performance and metabolic efficiency Prediction of burden-causing bottlenecks by genome-scale modeling can point to ways for optimizing the synthesis of desired metabolites, thereby minimizing the unwanted
  • Impact of the Expression System on Recombinant Protein . . .
    Results showed that metabolic burden associated with transcription and translation of foreign genes involves a decrease in recombinant protein expression It is necessary to find a balance between plasmid copy number and promoter strength to maximize soluble recombinant protein expression
  • Development of expression vectors for Escherichia coli based . . .
    We have constructed a series of expression vectors based on the pCR2 replicon and demonstrated its high stability and sustained expression capacity, in the absence of selection pressure which will make it an efficient tool for metabolic engineering and co-expression studies, as well as for scale up of expression
  • Stability and compatibility of pCR2 replicon with p15 replicon. . .
    Recent developments in metabolic engineering and the need for expanded compatibility required for co-expression studies, underscore the importance of developing new plasmid vectors with
  • Impact of the Expression System on Recombinant Protein . . .
    Results showed that metabolic burden associated with transcription and translation of foreign genes involves a decrease in recombinant protein expression It is necessary to find a balance between plasmid copy number and promoter strength to maximize soluble recombinant protein expression
  • Metabolic Burden: Cornerstones in Synthetic Biology and . . .
    We discuss cell physiological responses to metabolic burdens, as well as strategies to identify and resolve the carbon and energy burden problems, including metabolic balancing, enhancing respiration, dynamic regulatory systems, chromosomal engineering, decoupling cell growth with production phases, and co-utilization of nutrient resources





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